The results of in vitro screening tests are often presented as an inhibitory concentration 50% (IC 50): the concentration of the tested agent that inhibits the proliferation of the cancer cell population to 50% of the theoretically possible effect (absolute IC 50) or maximum effect practically achieved by the drug (relative IC 50). Nowadays, this approach, with various variations has also been adopted in a number of laboratories working in the area of anticancer drug development. Despite some criticism, the validity of this approach has been demonstrated by the results of almost 2 decades of extensive use. Sixty human cancer cell lines representing 9 types of tumors were selected as the test panel (NCI60 cancer cell line panel). National Cancer Institute (NCI) in 1990 ( ). The first large-scale in vitro screening program was launched by the U.S. This approach was developed in the late 1980s and evolved from earlier screening programs based on in vivo mouse leukemia models. This automatically derivable index also corresponds neatly to the use of complete and partial responses and tumor stability data generated in human tumors, and can be used to assess the efficacy of interventions to be used in clinical studies.High-throughput in vitro screening of natural and synthetic compounds is an important first stage in the selection of new anticancer drugs throughout the world. This index, comprised of three distinct values, the Tumor Inhibition Score, Tumor Rejection Score, and Tumor Stability Score, provides a complete picture of nearly every aspect of tumor growth in large numbers of animals, can be deduced automatically from tumor diameter or volume data, and can be used to compare several groups of animals in different experiments. Here, using tumor growth data from a large number of mice challenged with live tumor cells, we describe the use of a new composite parameter, Tumor Control Index (TCI) as an alternative method to do the same. Measurement of tumor diameters, tumor volumes, or area under the curve has been traditionally used to quantitate and compare tumor growth curves in immune competent as well as immune-compromised mice and rats.
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